Electrochemistry of catalase at a liquid|liquid micro-interface array

Abstract The electrochemistry of catalase (CAT) was investigated at the interface between two immiscible electrolyte solutions (ITIES) as a step towards its detection. Electrochemistry ITIES offers advantages such non-redox detection biomolecules. electrochemical behaviour CAT ITIES, in micro-interface array format, displayed distinct cyclic voltammogram when aqueous phase pH lower than isoelectric point (pI) CAT. No voltammetric response observed pH > pI CAT, indicating that neutral or negatively charged has no capability to facilitate anion transfer from organic phase. Adsorptive stripping voltammetry (AdSV) assessed for low concentrations µITIES array. Application positive preconcentration potential fixed time enabled interfacial accumulation complex; subsequently, scan potentials desorbed complex, providing electroanalytical signal. Assessment sample matrix effects by examining artificial serum indicated pH-adjusted samples is feasible. Together, these results demonstrate electroactive liquid–liquid and this may be useful strategy detect characterize enzyme label-free manner.